Via 7th Space, a study on mosquitoes in Brazil :
" Dengue epidemics have been reported in Brazil since 1985. The scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of DHF/DSS cases as well as the number of fatalities.
Herein, we report dengue virus surveillance in mosquitoes using a Flavivirus genus-specic RT-Hemi-Nested-PCR assay. The mosquitoes (Culicidae, n=1700) collected in the Northeast, Southeast and South of Brazil, between 1999 and 2005, were grouped into 154 pools.Putative genomes of DENV-1, -2 and -3 were detected in 6 mosquito pools (3.8%).
One amplicon of putative DENV-1 was detected in a pool of Haemagogus leucocelaenus suggesting that this virus could be involved in a sylvatic cycle."
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