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Thursday, September 1, 2011

An H5N1 highly pathogenic avian influenza virus isolated from a local tree sparrow in Indonesia

From Ida at Bird Flu Information Corner, excerpt :

" In May 2010, a few tree sparrows were found debilitated in Sidoarjo City in East Java, Indonesia. In this area, there are no commercial chicken farms, but some traditional duck farms existed. Also, it is near the coast, where many waterfowls gather. The sparrows exhibited clinical symptoms, with central nervous signs, depression, and torticollis.

Hemorrhage in the lung and brain was found at necropsy. A mixture of trachea, lungs, and colon from one of these sparrows was homogenized in 10 times volume of phosphate-buffered saline containing 5000 units/mL of penicillin and 5 mg/mL of streptomycin. The supernatant of the homogenate was inoculated into 10-day-old embryonated eggs, and after a 2-day incubation at 37°C, chorioallantoic fluid was harvested. The HA subtype was identified by using the hemagglutinin inhibition assay with anti-H5 chicken serum (Pusvetma, Surabaya, Indonesia)."

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